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medium high glucose dmem hg  (Thermo Fisher)


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    Structured Review

    Thermo Fisher medium high glucose dmem hg
    Medium High Glucose Dmem Hg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/medium+high+glucose+dmem+hg/D(%2B)-Glucose/pmc12686952-46-7-10
    Average 99 stars, based on 1 article reviews
    medium high glucose dmem hg - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Modification:

    Article Title: Brazilein inhibits epithelial-mesenchymal transition (EMT) and programmed death ligand 1 (PD-L1) expression in breast cancer cells.
    Article Snippet: Triple-negative breast cancer (TNBC) exhibits high levels of Epithelial-mesenchymal transition (EMT) and Programmed death ligand 1 (PD-L1) expression, which promotes immune escape and metastasis.. Brazilein is a natural compound extracted from Caesalpinia sappan L., and has been demonstrated to be an anti-inflammatory antiproliferative and apoptosis-inducer in various cancer cells.. Here, we investigated the effect of brazilein on EMT and PD-L1 expression in breast cancer cells and its related molecular mechanisms using MCF-7 and MDAMB-231 cells as a model.

    Article Title: SARS-CoV-2 inhibition through mRNA delivery using engineered extracellular vesicles displaying the spike protein.
    Article Snippet: Flp-In-293 cells (HEK293 cells) were purchased from Thermo Fisher Scientific. .. HEK293 cells and HEK293-CD63-HiBiT cells were cultured in Dulbecco’s modified Eagle’s medium high glucose (DMEM-HG) containing 10 % (v/v) heat-inactivated fetal bovine serum (FBS) (Gibco) [DMEM-HG(+)]. ..

    Article Title: Artificially inserted G-quadruplex DNA secondary structures induce long-distance chromatin activation
    Article Snippet: .. HEK 293T cells were cultured in Dulbecco’s Modified Eagle’s Medium-High Glucose (DMEM-HG) supplemented with 10% FBS and 1XAnti-Anti (Gibco). .. Histone H3 rabbit polyclonal (Abcam ab1791), H3K4Me1 rabbit polyclonal (Abcam ab8895), H3K27Ac rabbit polyclonal (Abcam ab4729), H3K4Me3 mouse monoclonal (Abcam ab1012), H3K27Me3 mouse monoclonal (Abcam ab6002), H3K9Me3 rabbit polyclonal (Abcam ab8848), p300 rabbit monoclonal (CST 54062), Ac-p300/CBP rabbit polyclonal (CST 4771), TRF2 rabbit polyclonal (Novus NB110-57130).

    Article Title: Synergistic effects of doxorubicin loaded silk fibroin nanoparticles and Cu-TiO 2 nanoparticles for local chemo-sonodynamic therapy against breast cancer.
    Article Snippet: A promising new approach to mitigate the adverse effects of chemotherapeutic drugs on healthy tissues involves combining sonodynamic therapy with topical chemotherapy to enhance the therapeutic efficacy of anticancer drugs.. In this study, we introduce a multi-functional in situ chitosan hydrogel (CS) containing silk fibroin nanoparticles (SFNPs) loaded with doxorubicin (DOXSFNPs) and CuO/TiO2 nanoparticles (CTNPs) for combination therapy.. The developed DOXSFNPs exhibited a size of 257 ± 6 nm, a zeta potential of − 14.3 ± 1.8 mV, and a high loading capacity of 12.38 ± 1.73 %.

    Article Title: Oxidative Stress Influences Exosome Biogenesis by Inducing Cellular Senescence in Uterine Adenocarcinoma Ishikawa Cells
    Article Snippet: The Ishikawa cells were obtained from the Stem Cell Research Center, an affiliated research center of Tabriz University of Medical Sciences. .. Cells were cultured in Dulbecco’s Modified Eagle’s Medium-High Glucose (DMEM-HG) (Gibco, USA) with 10% fetal bovine serum (FBS) (Gibco, USA) and 1% Penicillin-streptomycin (Pen-Strep) (Gibco, USA) solution. ..

    Article Title: Gallate Moiety of Catechin Is Essential for Inhibiting CCL2 Chemokine-Mediated Monocyte Recruitment.
    Article Snippet: Leukocyte recruitment witnesses an orchestrated complex formation between the chemokines and their molecular partners.. CCL2 chemokine that regulates monocyte trafficking is a worthwhile system from the pharmaceutical perspective.. In the current study, four major catechins (EC/EGC/ECG/EGCG) were assessed for their inhibitory potential against CCL2-regulated monocyte/macrophage recruitment.

    Article Title: Comparison of transcriptome profiles of radiotherapy beams on MCF-7 breast cancer xenografts.
    Article Snippet: In this study, MCF-7 (ATCC HTB-22) breast cancer cells for cell culture procedures were provided by the American Type Culture Collection (ATCC). .. These cells were then incubated in Dulbecco’s Modified Eagle’s Medium High Glucose (DMEM-HG) (GibcoTM) medium, which contained a final concentration of 2% penicillin/streptomycin (Sigma-Aldrich) and 10% fetal bovine serum (Sigma-Aldrich), at 37 °C with 5% CO2 for 24 h. The cells were passaged using trypsin (Sigma-Aldrich) when the cell culture dish reached 80% capacity. .. A total volume of 100 μl containing 1 × 107 cells suspended in a (1:1 mixture of DMEM-HG and Corning® Matrigel® Matrix (Cat. No.356255) was administered subcutaneously to each animal.

    Article Title: Artificially inserted strong promoter containing multiple G-quadruplexes induces long-range chromatin modification
    Article Snippet: .. The cells were cultured in Dulbecco’s Modified Eagle’s Medium- High Glucose (DMEM-HG) supplemented with 10% FBS and 1XAnti-Anti (Gibco). .. Histone H3 rabbit polyclonal (Abcam ab1791), H3K4Me1 rabbit polyclonal (Abcam ab8895), H3K27Ac rabbit polyclonal (Abcam ab4729), H3K4Me3 mouse monoclonal (Abcam ab1012), H3K27Me3 mouse monoclonal (Abcam ab6002), H3K9Me3 rabbit polyclonal (Abcam ab8898), p300 rabbit monoclonal (CST 54062), Ac-p300/CBP rabbit polyclonal (CST 4771), BG4 antibody (Sigma-Aldrich MABE917).

    Cell Culture:

    Article Title: SARS-CoV-2 inhibition through mRNA delivery using engineered extracellular vesicles displaying the spike protein.
    Article Snippet: Flp-In-293 cells (HEK293 cells) were purchased from Thermo Fisher Scientific. .. HEK293 cells and HEK293-CD63-HiBiT cells were cultured in Dulbecco’s modified Eagle’s medium high glucose (DMEM-HG) containing 10 % (v/v) heat-inactivated fetal bovine serum (FBS) (Gibco) [DMEM-HG(+)]. ..

    Article Title: Artificially inserted G-quadruplex DNA secondary structures induce long-distance chromatin activation
    Article Snippet: .. HEK 293T cells were cultured in Dulbecco’s Modified Eagle’s Medium-High Glucose (DMEM-HG) supplemented with 10% FBS and 1XAnti-Anti (Gibco). .. Histone H3 rabbit polyclonal (Abcam ab1791), H3K4Me1 rabbit polyclonal (Abcam ab8895), H3K27Ac rabbit polyclonal (Abcam ab4729), H3K4Me3 mouse monoclonal (Abcam ab1012), H3K27Me3 mouse monoclonal (Abcam ab6002), H3K9Me3 rabbit polyclonal (Abcam ab8848), p300 rabbit monoclonal (CST 54062), Ac-p300/CBP rabbit polyclonal (CST 4771), TRF2 rabbit polyclonal (Novus NB110-57130).

    Article Title: Synergistic effects of doxorubicin loaded silk fibroin nanoparticles and Cu-TiO 2 nanoparticles for local chemo-sonodynamic therapy against breast cancer.
    Article Snippet: A promising new approach to mitigate the adverse effects of chemotherapeutic drugs on healthy tissues involves combining sonodynamic therapy with topical chemotherapy to enhance the therapeutic efficacy of anticancer drugs.. In this study, we introduce a multi-functional in situ chitosan hydrogel (CS) containing silk fibroin nanoparticles (SFNPs) loaded with doxorubicin (DOXSFNPs) and CuO/TiO2 nanoparticles (CTNPs) for combination therapy.. The developed DOXSFNPs exhibited a size of 257 ± 6 nm, a zeta potential of − 14.3 ± 1.8 mV, and a high loading capacity of 12.38 ± 1.73 %.

    Article Title: Oxidative Stress Influences Exosome Biogenesis by Inducing Cellular Senescence in Uterine Adenocarcinoma Ishikawa Cells
    Article Snippet: The Ishikawa cells were obtained from the Stem Cell Research Center, an affiliated research center of Tabriz University of Medical Sciences. .. Cells were cultured in Dulbecco’s Modified Eagle’s Medium-High Glucose (DMEM-HG) (Gibco, USA) with 10% fetal bovine serum (FBS) (Gibco, USA) and 1% Penicillin-streptomycin (Pen-Strep) (Gibco, USA) solution. ..

    Article Title: Comparison of transcriptome profiles of radiotherapy beams on MCF-7 breast cancer xenografts.
    Article Snippet: In this study, MCF-7 (ATCC HTB-22) breast cancer cells for cell culture procedures were provided by the American Type Culture Collection (ATCC). .. These cells were then incubated in Dulbecco’s Modified Eagle’s Medium High Glucose (DMEM-HG) (GibcoTM) medium, which contained a final concentration of 2% penicillin/streptomycin (Sigma-Aldrich) and 10% fetal bovine serum (Sigma-Aldrich), at 37 °C with 5% CO2 for 24 h. The cells were passaged using trypsin (Sigma-Aldrich) when the cell culture dish reached 80% capacity. .. A total volume of 100 μl containing 1 × 107 cells suspended in a (1:1 mixture of DMEM-HG and Corning® Matrigel® Matrix (Cat. No.356255) was administered subcutaneously to each animal.

    Article Title: Artificially inserted strong promoter containing multiple G-quadruplexes induces long-range chromatin modification
    Article Snippet: .. The cells were cultured in Dulbecco’s Modified Eagle’s Medium- High Glucose (DMEM-HG) supplemented with 10% FBS and 1XAnti-Anti (Gibco). .. Histone H3 rabbit polyclonal (Abcam ab1791), H3K4Me1 rabbit polyclonal (Abcam ab8895), H3K27Ac rabbit polyclonal (Abcam ab4729), H3K4Me3 mouse monoclonal (Abcam ab1012), H3K27Me3 mouse monoclonal (Abcam ab6002), H3K9Me3 rabbit polyclonal (Abcam ab8898), p300 rabbit monoclonal (CST 54062), Ac-p300/CBP rabbit polyclonal (CST 4771), BG4 antibody (Sigma-Aldrich MABE917).

    Saline:

    Article Title: Synergistic effects of doxorubicin loaded silk fibroin nanoparticles and Cu-TiO 2 nanoparticles for local chemo-sonodynamic therapy against breast cancer.
    Article Snippet: A promising new approach to mitigate the adverse effects of chemotherapeutic drugs on healthy tissues involves combining sonodynamic therapy with topical chemotherapy to enhance the therapeutic efficacy of anticancer drugs.. In this study, we introduce a multi-functional in situ chitosan hydrogel (CS) containing silk fibroin nanoparticles (SFNPs) loaded with doxorubicin (DOXSFNPs) and CuO/TiO2 nanoparticles (CTNPs) for combination therapy.. The developed DOXSFNPs exhibited a size of 257 ± 6 nm, a zeta potential of − 14.3 ± 1.8 mV, and a high loading capacity of 12.38 ± 1.73 %.

    MTT Assay:

    Article Title: Synergistic effects of doxorubicin loaded silk fibroin nanoparticles and Cu-TiO 2 nanoparticles for local chemo-sonodynamic therapy against breast cancer.
    Article Snippet: A promising new approach to mitigate the adverse effects of chemotherapeutic drugs on healthy tissues involves combining sonodynamic therapy with topical chemotherapy to enhance the therapeutic efficacy of anticancer drugs.. In this study, we introduce a multi-functional in situ chitosan hydrogel (CS) containing silk fibroin nanoparticles (SFNPs) loaded with doxorubicin (DOXSFNPs) and CuO/TiO2 nanoparticles (CTNPs) for combination therapy.. The developed DOXSFNPs exhibited a size of 257 ± 6 nm, a zeta potential of − 14.3 ± 1.8 mV, and a high loading capacity of 12.38 ± 1.73 %.

    Incubation:

    Article Title: Comparison of transcriptome profiles of radiotherapy beams on MCF-7 breast cancer xenografts.
    Article Snippet: In this study, MCF-7 (ATCC HTB-22) breast cancer cells for cell culture procedures were provided by the American Type Culture Collection (ATCC). .. These cells were then incubated in Dulbecco’s Modified Eagle’s Medium High Glucose (DMEM-HG) (GibcoTM) medium, which contained a final concentration of 2% penicillin/streptomycin (Sigma-Aldrich) and 10% fetal bovine serum (Sigma-Aldrich), at 37 °C with 5% CO2 for 24 h. The cells were passaged using trypsin (Sigma-Aldrich) when the cell culture dish reached 80% capacity. .. A total volume of 100 μl containing 1 × 107 cells suspended in a (1:1 mixture of DMEM-HG and Corning® Matrigel® Matrix (Cat. No.356255) was administered subcutaneously to each animal.

    Concentration Assay:

    Article Title: Comparison of transcriptome profiles of radiotherapy beams on MCF-7 breast cancer xenografts.
    Article Snippet: In this study, MCF-7 (ATCC HTB-22) breast cancer cells for cell culture procedures were provided by the American Type Culture Collection (ATCC). .. These cells were then incubated in Dulbecco’s Modified Eagle’s Medium High Glucose (DMEM-HG) (GibcoTM) medium, which contained a final concentration of 2% penicillin/streptomycin (Sigma-Aldrich) and 10% fetal bovine serum (Sigma-Aldrich), at 37 °C with 5% CO2 for 24 h. The cells were passaged using trypsin (Sigma-Aldrich) when the cell culture dish reached 80% capacity. .. A total volume of 100 μl containing 1 × 107 cells suspended in a (1:1 mixture of DMEM-HG and Corning® Matrigel® Matrix (Cat. No.356255) was administered subcutaneously to each animal.



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